不用本金就能赚钱的方法

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现在位置不用本金就能赚钱的方法>技术资料不用本金就能赚钱的方法>产品信息>说明书>CSB-E04567h (中英文)人生长激素(GH)ELISA Kit

CSB-E04567h (中英文)人生长激素(GH)ELISA Kit

厦门慧嘉生物科技有限公司2013年4月18日 11:33 点击:824

不用本金就能赚钱的方法----------------------- Page 1-----------------------

  Human Growth Hormone(HGH)

                           ELISA KIT

 

不用本金就能赚钱的方法                      Catalog No. CSB-E04567h

 

                                    (96T)

 

    This immunoassay kit allows for the in vitro quantitative determination of human

 

不用本金就能赚钱的方法    HGH concentrations in serum, plasma and other biological fluids.

 

    Expiration date   six months from the date of manufacture

 

不用本金就能赚钱的方法    FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

 

不用本金就能赚钱的方法                                       1


不用本金就能赚钱的方法 ----------------------- Page 2-----------------------

INTRODUCTION

 

HGH      is  Human     Growth    Hormone,     a  natural   hormone      produced    in  the

 

不用本金就能赚钱的方法pituitary gland of the brain. HGH is considered "the key" hormone because

 

it controls so many functions. It’s responsible for youth, vitality, energy and

 

all   of   the   health   benefits   we   associate   with   youth.   Dr.   Daniel   Rudman’s

 

不用本金就能赚钱的方法study     in  the   New     England     Journal    Of   Medicine     demonstrated       the

 

remarkable ability to reverse the effects of aging upon the human body with

 

the   employment   of   HGH   -   Human   Growth   Hormone!   Due   in   part   to   his

 

efforts, Dr. Rudmans’s study saw the effects of HGH upon overweight men

 

不用本金就能赚钱的方法between the ages of 61 and 80 years of age.

 

不用本金就能赚钱的方法HGH reduces body fat The men did not alter their personal habits of eating,

 

smoking,      or  exercise,   yet  with   the  consumption      of  HGH,    they   lost  an

 

不用本金就能赚钱的方法average of 14% of their body fat, while gaining an average of 8.8% lean

 

muscle mass. Their skin became firmer and they experienced a localized

 

increase in bone density. Over all, HgH appeared to reverse the effects of

 

aging by 10-20 years!!! HGH is prescribed and administered by a doctor in

 

the form of injections. But a nonprescription form is also available over the

 

counter and through mail order.

 

HGH   promotes   growth   in   children   and   plays   an   important   role   in   adult

 

metabolism.      The    body   secretes    the   hormone,     in  decreasing     amounts,

 

throughout      our  lifetimes.  The    amount    of  hormone     in the   body    can   be

 

measured by levels of IGF-1 (Insulin Growth Factor). Growth hormone has

 

                                             2


----------------------- Page 3-----------------------

不用本金就能赚钱的方法a profound effect on all the cells of the body, more than any other hormone

 

不用本金就能赚钱的方法because it is the cell generator.

 

不用本金就能赚钱的方法HGH is the "master hormone" controlling many organs and body functions

 

and   is   directly   responsible   for   stimulating   tissue  repair,   cell   replacement,

 

不用本金就能赚钱的方法brain   functions,   and   enzyme   function!   It’s   human   growth   hormone   that

 

grows the cells, bones, muscles, and organs, and it is the decreasing level

 

of human growth hormone after age 30 that slowly robs us of our "youth."

 

不用本金就能赚钱的方法PRINCIPLE OF THE ASSAY

 

The microtiter plate provided in this kit has been pre-coated with biotin-BSA

 

不用本金就能赚钱的方法and    Avidin.   Standards    or  samples     are  then   added    to the   appropriate

 

microtiter plate wells with a biotin-conjugated HGH antibody and incubated.

 

Then     Horseradish     Peroxidase     (HRP)-conjugated        antibody    preparation

 

不用本金就能赚钱的方法specific for HGH are added and incubated. Substrate solutions are added to

 

不用本金就能赚钱的方法each well. The enzyme-substrate reaction is terminated by the addition of a

 

sulphuric      acid    solution     and     the    color    change      is   measured

 

spectrophotometrically        at  a   wavelength     of   450   nm    ±  2    nm.   The

 

concentration of HGH in the samples is then determined by comparing the

 

不用本金就能赚钱的方法O.D. of the samples to the standard curve.

 

不用本金就能赚钱的方法DETECTION RANGE

 

2.5 ng/ml-50 ng/ml. The standard curve concentrations used for the ELISA’s

 

不用本金就能赚钱的方法were 50 ng/ml, 25ng/ml,10 ng/ml, 5 ng/ml,2.5 ng/ml.

 

                                           3


----------------------- Page 4-----------------------

SPECIFICITY

 

不用本金就能赚钱的方法This   assay   recognizes    human    HGH.    No  significant  cross-reactivity  or

 

interference was observed.

 

SENSITIVITY

 

不用本金就能赚钱的方法The minimum detectable dose of human HGH is typically less than 1 ng/ml.

 

The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined

 

as the lowest protein concentration that could be differentiated from zero.

 

不用本金就能赚钱的方法MATERIALS PROVIDED

 

              Reagent                                    Quantity

 

不用本金就能赚钱的方法              Assay plate                                     1

 

              Standards (S1-S4)                           5x 1 ml

 

不用本金就能赚钱的方法              Biotin-antibody                             1 x 6 ml

 

              HRP-conjugate                              1 x 10 ml

 

                                                         1 x 20 ml

              Wash Buffer

不用本金就能赚钱的方法                                                     (10×concentrate)

 

              Substrate A                                 1 x 7 ml

 

              Substrate B                                 1 x 7 ml

 

              Stop Solution                               1 x 7 ml

 

不用本金就能赚钱的方法   Standard       Standard1    Standard2    Standard3     Standard4    Standard5

 

Concentration

                     2.5             5           10           25            50

不用本金就能赚钱的方法    (ng/ml)

 

                                        4


不用本金就能赚钱的方法 ----------------------- Page 5-----------------------

STORAGE

 

不用本金就能赚钱的方法1.   Unopened   test   kits   should   be   stored   at   2-8°C   upon   receipt   and   the

 

    microtiter plate should be kept in a sealed bag. The test kit may be used

 

不用本金就能赚钱的方法    throughout the expiration date of the kit. Refer to the package label for

 

    the expiration date.

 

2.   Opened     test  kits  will  remain   stable   until  the  expiring   date   shown,

 

    provided it is stored as prescribed above.

 

3.   A   microtiter   plate   reader   with   a   bandwidth   of   10   nm   or   less   and   an

 

    optical   density   range   of   0-3   OD   or   greater   at   450nm   wavelength   is

 

    acceptable for use in absorbance measurement.

 

REAGENT PREPARATION

 

Bring all reagents to room temperature before use.

 

1.   Wash Buffer        If crystals have formed in the concentrate, warm up to

 

不用本金就能赚钱的方法     room   temperature   and   mix   gently   until   the   crystals  have   completely

 

     dissolved. Dilute 20 ml of Wash Buffer Concentrate  into deionized or

 

     distilled water to prepare 200 ml of Wash Buffer.

 

不用本金就能赚钱的方法Precaution: The Stop Solution provided with this kit is an acid solution. Wear

 

不用本金就能赚钱的方法            eye, hand, face, and clothing protection when using this material.

 

OTHER SUPPLIES REQUIRED

 

      Microplate   reader   capable   of   measuring   absorbance  at   450   nm,   with

 

     the correction wavelength set at 540 nm or 570 nm.

 

不用本金就能赚钱的方法      Pipettes and pipette tips.

 

      Deionized or distilled water.

 

不用本金就能赚钱的方法      Squirt bottle, manifold dispenser, or automated microplate washer.

 

                                            5


不用本金就能赚钱的方法 ----------------------- Page 6-----------------------

不用本金就能赚钱的方法SAMPLE COLLECTION AND STORAGE

 

不用本金就能赚钱的方法     Serum      Use a serum separator tube (SST) and allow samples to clot

 

     for 30 minutes before centrifugation for 15 minutes at 1000 g. Remove

 

     serum and assay immediately or aliquot and store samples at -20° C.

 

     Avoid repeated freeze-thaw cycles.

 

不用本金就能赚钱的方法     Plasma       Collect    plasma     using   citrate,  EDTA,     or  heparin    as   an

 

     anticoagulant. Centrifuge for 15 minutes at 1000 g within 30 minutes of

 

     collection.   Assay   immediately   or   aliquot   and   store  samples   at   -20°C.

 

不用本金就能赚钱的方法     Avoid repeated freeze-thaw cycles.

 

Note: Grossly hemolyzed samples are not suitable for use in this assay.

 

ASSAY PROCEDURE

 

不用本金就能赚钱的方法Bring    all  reagents   and   samples     to  room    temperature   before    use.   It  is

 

recommended that all samples, standards, and controls be assayed in duplicate.

 

1.    Add    50μl   of Standard      or  Sample       per  well.   Then    add   50μl    of

 

     Biotin-antibody to   each   well.   Standards   need   test   in   duplicate.   Mix

 

     well and then incubate for 30 min at 37°C.

 

2.    Fill each well with Wash Buffer (about 200μl), stay for 10 seconds and

 

不用本金就能赚钱的方法     Spinning.   Repeat   the   process   for   a   total   of   three   washes.   Complete

 

不用本金就能赚钱的方法     removal of liquid at each step is essential to good performance. After

 

     the   last   wash,   remove   any   remaining   Wash   Buffer   by  aspirating   or

 

     decanting. Invert the plate and blot it against clean paper towels.

 

                                            6


不用本金就能赚钱的方法 ----------------------- Page 7-----------------------

不用本金就能赚钱的方法3.    Add 50μl of HRP-conjugate to each well. Mix well and then incubate for

 

     30 min at 37°C.

 

4.    Wash the plate as before.

 

5.    Add 50μl of Substrate A and 50μl of Substrate B to each well, mix well.

 

     Incubate for 15 minutes at 18-25°C. Keeping the plate away from drafts

 

     and other temperature fluctuations in the dark.

 

6.    Add   50μl   of  Stop   Solution   to   each   well.   If   color   change   does   not

 

     appear uniform, gently tap the plate to ensure thorough mixing.

 

7.    Determine the optical density of each well within 10 minutes, using a

 

     microplate reader set to 450 nm.

 

CALCULATION OF RESULTS

 

不用本金就能赚钱的方法Average the duplicate readings for each standard, control, and sample and

 

不用本金就能赚钱的方法subtract the average zero standard optical density. Create a standard curve

 

不用本金就能赚钱的方法by reducing the data using computer software capable of generating a four

 

parameter logistic (4-PL) curve-fit. As an alternative, construct a standard

 

curve   by   plotting   the   mean   absorbance   for   each   standard   on   the   y-axis

 

不用本金就能赚钱的方法against the concentration on the x-axis and draw a best fit curve through the

 

points on the graph. The data may be linearized by plotting the log of the

 

HGH concentrations versus the log of the O.D. and the best fit line can be

 

determined       by   regression    analysis.    This   procedure      will  produce    an

 

adequate but less precise fit of the data. If samples have been diluted, the

 

concentration      read   from   the  standard    curve   must    be multiplied    by   the

 

dilution factor.

 

                                             7


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不用本金就能赚钱的方法LIMITATIONS OF THE PROCEDURE

 

不用本金就能赚钱的方法     The kit should not be used beyond the expiration date on the kit label.

 

     Do not mix or substitute reagents with those from other lots or sources.

 

     It is important that the Calibrator Diluent selected for the standard curve

 

    be consistent with the samples being assayed.

 

     If samples generate values higher than the highest standard, dilute the

 

    samples with the appropriate Calibrator Diluent and repeat the assay.

 

     Any    variation   in  Standard     Diluent,   operator,    pipetting   technique,

 

不用本金就能赚钱的方法    washing   technique,   incubation   time   or   temperature,  and   kit   age   can

 

不用本金就能赚钱的方法    cause variation in binding.

 

     This assay is designed to eliminate interference by soluble receptors,

 

不用本金就能赚钱的方法    binding proteins, and other factors present in biological samples. Until

 

    all   factors  have    been   tested   in  the  Quantikine    Immunoassay,       the

 

不用本金就能赚钱的方法    possibility of interference cannot be excluded.

 

不用本金就能赚钱的方法TECHNICAL HINTS

 

      When mixing or reconstituting protein solutions, always avoid foaming.

 

不用本金就能赚钱的方法      To avoid cross-contamination, change pipette tips between additions of

 

不用本金就能赚钱的方法     each standard level, between sample additions, and between reagent

 

     additions. Also, use separate reservoirs for each reagent.

 

不用本金就能赚钱的方法      When   using   an   automated   plate   washer,   adding   a   30  second   soak

 

不用本金就能赚钱的方法     period   following   the   addition   of   wash   buffer,   and/or   rotating   the   plate

 

不用本金就能赚钱的方法     180 degrees between wash steps may improve assay precision.

 

                                           8


----------------------- Page 9-----------------------

      To   ensure   accurate   results,   proper   adhesion   of   plate   sealers   during

 

     incubation steps is necessary.

 

      Substrate   Solution   should   remain   colorless   until   added   to   the   plate.

 

     Keep Substrate Solution protected from light. Substrate Solution should

 

不用本金就能赚钱的方法     change from colorless to gradations of blue.

 

      Stop Solution should be added to the plate in the  same order as the

 

不用本金就能赚钱的方法     Substrate Solution. The color developed in the wells will turn from blue

 

     to   yellow   upon   addition   of   the   Stop   Solution. Wells  that   are   green   in

 

不用本金就能赚钱的方法     color indicate that the Stop Solution has not mixed thoroughly with the

 

不用本金就能赚钱的方法     Substrate Solution.

 

                                              9


----------------------- Page 10-----------------------

                  RENSHENGZHANGJISURENSHENGZHANGJISU(GH)MEILIANMIANYIFENXIMEILIANMIANYIFENXI

不用本金就能赚钱的方法                  RENSHENGZHANGJISURENSHENGZHANGJISU             MEILIANMIANYIFENXIMEILIANMIANYIFENXI

 

                          SHIJIHESHIYONGSHUOMINGSHUSHIJIHESHIYONGSHUOMINGSHU

                          SHIJIHESHIYONGSHUOMINGSHUSHIJIHESHIYONGSHUOMINGSHU

 

不用本金就能赚钱的方法BENSHIJIHEJINGONGYANJIUSHIYONGBENSHIJIHEJINGONGYANJIUSHIYONG

BENSHIJIHEJINGONGYANJIUSHIYONGBENSHIJIHEJINGONGYANJIUSHIYONG

 

不用本金就能赚钱的方法CHANPINBIANHAOCHANPINBIANHAO::CSB-E04567h

CHANPINBIANHAOCHANPINBIANHAO::

 

JIANCEFANWEIJIANCEFANWEI::2.5 ng/ml – 50 ng/ml

JIANCEFANWEIJIANCEFANWEI::

 

ZUIDIJIANCEXIANZUIDIJIANCEXIAN::1 ng/ml

不用本金就能赚钱的方法ZUIDIJIANCEXIANZUIDIJIANCEXIAN::

 

TEYIXINGTEYIXING::BENSHIJIHEKEJIANCERENHGH,QIEYUQITAXIANGGUANDANBAIWUJIAOCHAFANYING。

TEYIXINGTEYIXING::

 

不用本金就能赚钱的方法YOUXIAOQIYOUXIAOQI::6 GEYUE

YOUXIAOQIYOUXIAOQI::

 

YUQIYINGYONGYUQIYINGYONG::ELISA FADINGLIANGCEDINGRENXUEQING、XUEJIANGHUOQITAXIANGGUANSHENGWUYETIZHONGHGH

YUQIYINGYONGYUQIYINGYONG::

 

HANLIANG。

 

SHUOMINGSHUOMING

SHUOMINGSHUOMING

 

不用本金就能赚钱的方法1.  NONGXIDIYEDIWENBAOCUNHUIYOUYANXICHU,XISHISHIKEZAISHUIYUZHONGJIAWENZHURONG。

 

2.  GANGKAIQIDEMEILIANBANKONGZHONGKENENGHUIHANYOUSHAOXUSHUIYANGWUZHI,CIWEIZHENGCHANGXIANXIANG,BUHUIDUISHI

 

    YANJIEGUOZAOCHENGRENHEYINGXIANG。

 

SHIYANYUANLISHIYANYUANLI

SHIYANYUANLISHIYANYUANLI

 

     YONGSHENGWUSUHUANIUXUEQINGBAIDANBAIHEQINHESUBAOBEIWEIKONGBAN,ZHICHENGGUXIANGZAITI,JIANGYIZHI

 

NONGDUDEHGH DEBIAOZHUNPIN、BIAOBENJIARUWEIKONGBANZHONG,SHIQIYUSHENGWUSUBIAOJIDEHGH KANGTI

 

不用本金就能赚钱的方法TONGSHIWENYU,XIDIHOU,JIARULAGENGUOYANGHUAWUMEIBIAOJIDEKANGTI,ZAIJINGGUOWENYUHEXIDIHOU

 

YONGDIWUXIANSE。YANSEDESHENQIANHEYANGPINZHONGDEHGH  DENONGDUCHENGBILIGUANXI。YONGMEIBIAOYIZAI

 

450nm BOZHANGXIACEDINGXIGUANGDU (OD ZHI),JISUANYANGPINNONGDU。

 

                                      10


----------------------- Page 11-----------------------

SHIJIHEZUCHENGJISHIJIPEIZHISHIJIHEZUCHENGJISHIJIPEIZHI

不用本金就能赚钱的方法SHIJIHEZUCHENGJISHIJIPEIZHISHIJIHEZUCHENGJISHIJIPEIZHI

 

1.  MEILIANBANMEILIANBAN(Assay plate ):                                      YIKUAI(96 KONG)。

不用本金就能赚钱的方法    MEILIANBANMEILIANBAN

 

2.  BIAOZHUNPINBIAOZHUNPIN(Standard):                                             5×1ml/PING。

不用本金就能赚钱的方法    BIAOZHUNPINBIAOZHUNPIN

 

 Standard 1       Standard 2      Standard 3    Standard 4       Standard 5

 

  2.5 ng/ml         5 ng/ml        10ng/ml        25 ng/ml        50 ng/ml

 

不用本金就能赚钱的方法3.  MEIJIEHEWUMEIJIEHEWU(HRP-Conjugate):                                     1×10ml/PING。

    MEIJIEHEWUMEIJIEHEWU

 

4.  SHENGWUSUKANGTISHENGWUSUKANGTI((Biotin-antibody))                                 1×6ml/PING。

    SHENGWUSUKANGTISHENGWUSUKANGTI((                   ))

 

不用本金就能赚钱的方法5.  DIWURONGYEDIWURONGYEA ((Substrate A):)                                    1×7ml/PING。

    DIWURONGYEDIWURONGYE ((               ))

 

6.  DIWURONGYEDIWURONGYEB ((Substrate B):)                                    1×7ml/PING。

不用本金就能赚钱的方法    DIWURONGYEDIWURONGYE ((               ))

 

7.  NONGXIDIYENONGXIDIYE((Wash Buffer1×20ml/PING SHIYONGSHIMEIPINGYONGZHENGLIUSHUIRONGJIEDAO500ml。

    NONGXIDIYENONGXIDIYE((

 

8.  ZHONGZHIYEZHONGZHIYE((Stop Solution):)                                      1×7ml/PING。

不用本金就能赚钱的方法    ZHONGZHIYEZHONGZHIYE((                ))

 

XUYAOERWEITIGONGDESHIJIHEQICAIXUYAOERWEITIGONGDESHIJIHEQICAI

不用本金就能赚钱的方法XUYAOERWEITIGONGDESHIJIHEQICAIXUYAOERWEITIGONGDESHIJIHEQICAI

 

1.  BIAOZHUNGUIGEMEIBIAOYI

 

不用本金就能赚钱的方法2.  GAOSULIXINJI

 

3.  DIANREHENGWENPEIYANGXIANG

 

不用本金就能赚钱的方法4.  GANJINGDESHIGUANHEEppendof GUAN

 

5.  XILIEKEDIAOJIEYIYEQIJIXITOU,YICIJIANCEYANGPINJIAODUOSHI,ZUIHAOYONGDUOTONGDAOYIYEQI

 

6.  ZHENGLIUSHUI,RONGLIANGPINGDENG

 

BIAOBENDECAIJIJIBAOCUNBIAOBENDECAIJIJIBAOCUN

不用本金就能赚钱的方法BIAOBENDECAIJIJIBAOCUNBIAOBENDECAIJIJIBAOCUN

 

1.  XUEQING:QUANXUEBIAOBENQINGYUSHIWENFANGZHI2 XIAOSHIHUO4℃GUOYEHOUYU1000g LIXIN20 FENZHONG,

 

不用本金就能赚钱的方法    QUSHANGQINGJIKEJIANCE,HUOJIANGBIAOBENFANGYU-20℃HUO-80℃BAOCUN,DANYINGBIMIANFANFUDONGRONG。

 

                                     11


不用本金就能赚钱的方法 ----------------------- Page 12-----------------------

2.  XUEJIANG:KEYONGEDTA HUOGANSUZUOWEIKANGNINGJI,BIAOBENCAIJIHOU30 FENZHONGNEIYU2   -   8°C

 

    1000 g LIXIN15 FENZHONG,HUOJIANGBIAOBENFANGYU-20℃HUO-80℃BAOCUN,DANYINGBIMIANFANFUDONG

 

不用本金就能赚钱的方法    RONG。

 

不用本金就能赚钱的方法ZHU:ZHU:YISHANGBIAOBENZHIYISHANGBIAOBENZHI4℃℃BAOCUNYINGXIAOYUBAOCUNYINGXIAOYU1 ZHOU,ZHOU,-20℃℃HUOHUO-80℃℃JUNYINGMIFENGBAOCUNJUNYINGMIFENGBAOCUN,,-20℃℃BUYINGCHAOGUOBUYINGCHAOGUO1 GEGE

ZHUZHU::YISHANGBIAOBENZHIYISHANGBIAOBENZHI ℃℃BAOCUNYINGXIAOYUBAOCUNYINGXIAOYU ZHOUZHOU,, ℃℃HUOHUO ℃℃JUNYINGMIFENGBAOCUNJUNYINGMIFENGBAOCUN,, ℃℃BUYINGCHAOGUOBUYINGCHAOGUO GEGE

YUE,YUE,-80℃℃BUYINGCHAOGUOBUYINGCHAOGUO2  GEYUEGEYUE;BIAOBENRONG;BIAOBENRONGXUEHUIYINGXIANGZUIHOUJIANCEJIEGUOXUEHUIYINGXIANGZUIHOUJIANCEJIEGUO,YINCIRONGXUEBIAOBENBUYIJINXINGJIAN,YINCIRONGXUEBIAOBENBUYIJINXINGJIAN

YUEYUE,, ℃℃BUYINGCHAOGUOBUYINGCHAOGUO GEYUEGEYUE;;BIAOBENRONGBIAOBENRONGXUEHUIYINGXIANGZUIHOUJIANCEJIEGUOXUEHUIYINGXIANGZUIHOUJIANCEJIEGUO,,YINCIRONGXUEBIAOBENBUYIJINXINGJIANYINCIRONGXUEBIAOBENBUYIJINXINGJIAN

CE。CE。

CECE。。

 

CAOZUOBUZHOUCAOZUOBUZHOU

CAOZUOBUZHOUCAOZUOBUZHOU

 

1.  JIANGGEZHONGSHIJIZHISHIWEN 〔18-25℃〕PINGHENGBANXIAOSHI,QUNONGSUOXIDIYE,GENJUDANGPIJIAN

 

    CESHULIANG,YONGZHENGLIUSHUISHANG1:10 XISHI,HUNYUNHOUBEIYONG。

 

2.  JIANGMEIBIAOBANQUCHU,FENBIEXIANGKONGZHONGJIARU50ul BIAOZHUNPIN、BIAOBEN,ZAIFENBIEJIARU50ul SHENG

 

    WUSUHUAKANGTI,ZHENDONG10-20 MIAOHUNYUN,ZHI37℃FUYU30 FENZHONG。

 

不用本金就能赚钱的方法3.  SHOUGONGXIBAN,QIQUKONGNEIYETI。XIDIYEZHUMANGEKONG,JINGZHI10 MIAOSHUAIGAN,ZHONGFUSANCI

 

不用本金就能赚钱的方法    HOUPAIGAN;XIBANJIXIBAN,XUANZEXIDISANCICHENGXU,XIBANHOUPAIGAN。

 

不用本金就能赚钱的方法4.  MEIKONGJIARU100ul MEIJIEHEWU,ZHENDONG10-20 MIAOHUNYUN,ZHI37℃FUYU30 FENZHONG。

 

5.  SHOUGONGXIBAN,QIQUKONGNEIYETI。XIDIYEZHUMANGEKONG,JINGZHI10 MIAOSHUAIGAN,ZHONGFUSANCI

 

不用本金就能赚钱的方法    HOUPAIGAN;XIBANJIXIBAN,XUANZEXIDISANCICHENGXU,XIBANHOUPAIGAN。

 

6.  MEIKONGJIAXIANSEJIA YE50μl,XIANSEJIB YE50μl,ZHENDANGHUNYUNHOU,18-25℃BIGUANG

 

不用本金就能赚钱的方法    XIANSE15 FENZHONG,MEIKONGJIAZHONGZHIYE50μl。

 

7.  YONGMEIBIAOYIDUSHU,QUBOZHANG450nm,XIANYONGKONGBAIKONGDIAOLINGDIAN,RANHOUCEDINGGEKONGOD

 

    ZHI。

 

SHUJUCHULISHUJUCHULI

SHUJUCHULISHUJUCHULI

 

不用本金就能赚钱的方法1.  SHOUGONGZUOTU:YONGSHUANGDUISHUZUOBIAOZHI,YIBIAOZHUNPINNONGDUWEIHENGZHOU,YIDUIYINGDE0D ZHIWEIZONG

 

    ZHOU,HUACHUPINGHUAQUXIANHUOZHIXIAN,ZAIQUXIANSHANGANZHAODAICEXUEQINGOD ZHIZHAODAODUIYINGDENONGDU

 

不用本金就能赚钱的方法    ZHI。

 

2.  JISUANJI:SHIYONGXIANXINGNIHEGONGNENG,YINGJIANGBIAOZHUNPINS1-S5  DENONGDUQUDUISHU(Log(NONG

 

    DU))ZUOWEIX,JIANGDUIYINGDEOD ZHIJIANQUKONGBAIDUIZHAOKONGOD ZHIHOUQUDUISHU(Log(OD

 

不用本金就能赚钱的方法    ZHI-NSB))ZUOWEIY,JINXINGXIANXINGNIHE。ZAICONGNIHEXIANSHANGJISUANCHUDAICEXUEQINGNONGDU。

 

                                      12


----------------------- Page 13-----------------------

ZHUYISHIXIANGZHUYISHIXIANG

ZHUYISHIXIANGZHUYISHIXIANG

 

1.  CONGLENGZANGHUANJINGZHONGQUCHUDESHIJIHENEIQUANBUPINGZHUANGSHIJIJISUOXUYUBAOBEIBANTIAOYINGZHISHIWEN

 

不用本金就能赚钱的方法 (18-25℃)PINGHENG30 FENZHONGHOUFANGKESHIYONG,YUZHEYINGJISHIFENGHAOKOU,FANGHUI2-8℃ZHONGBI

 

GUANGBAOCUN,YIBEIHOUYONG。

 

2.  SHIYONGQIANSHIJIYINGYAOYUN。

 

3.  JIEGUOPANDUANXUZAIFANYINGZHONGZHIHOU10 FENZHONGNEIWANCHENG。

 

不用本金就能赚钱的方法4.  BUTONGPIHAODESHIJIBUKEHUNYONG。

 

5.  JIAYANGSHIYINGZHUYIBIMIANSUOYONGGESHIJIJIYANGPINZHIJIANDEJIAOYOUWURAN。

 

6.  CAOZUOSHI,SHIJIHENEIMEIZHONGSHIJIGESHIYONGYIGEXITOU,MEIYIZHONGBIAOZHUNPINSHIYONGYIGEXITOU,

 

    MEIYIGEYANGPINGESHIYONGYIGEXITOU,XITOUZUIHAOYICIXINGSHIYONG。

 

7.  MEICICESHIBIXUZHONGXINZHIZUOBIAOZHUNQUXIAN,SHANGCISHIYANBIAOZHUNQUXIANBUKEZHONGFUSHIYONG。

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