不用本金就能赚钱的方法

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CSB-E04577h(中英文)人γ干扰素(IFN-γ) elisa kit

厦门慧嘉生物科技有限公司2013年4月18日 11:35 点击:913

不用本金就能赚钱的方法----------------------- Page 1-----------------------

不用本金就能赚钱的方法         Human Interferon γ (IFN-γ)

                             ELISA Kit

 

不用本金就能赚钱的方法                         Catalog No. CSB-E04577h

 

不用本金就能赚钱的方法                                       (96T)

 

    This immunoassay kit allows for the in vitro quantitative determination of human IFN-γ

 

不用本金就能赚钱的方法    concentrations in cell culture supernates, serum, plasma and other biological fluids.

 

不用本金就能赚钱的方法    Expiration date    six months from the date of manufacture

 

不用本金就能赚钱的方法    FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

 

不用本金就能赚钱的方法                            CUSABIO BIOTECH CO., Ltd.

 

                    

 

                  E-mail: cusabio@cusabio.com  cusabio@cusabio.cn

 

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不用本金就能赚钱的方法INTRODUCTION

 

不用本金就能赚钱的方法Interferon-gamma (IFN-γ) is a dimerized soluble cytokine that is the only member

 

of    the   type    II  class    of  interferons.     This    interferon     was    originally    called

 

不用本金就能赚钱的方法macrophage-activating           factor.   The   IFN-γ  monomer         consists    of  a   core   of  six

 

不用本金就能赚钱的方法α-helices   and   an   extended   unfolded   sequence   in   the   C-terminal   region.   This   is

 

不用本金就能赚钱的方法shown in the structural models below. The α-helices in the core of the structure are

 

不用本金就能赚钱的方法numbered 1 to 6. Full length IFN-γ is 143 amino acids in length, the models are

 

126   amino   acids   in   length.   Affinity   for   the   glycosaminoglycan   heparan   sulfate

 

resides    solely    within   the   deleted    sequence     of  17   amino     acids.   In  contrast   to

 

不用本金就能赚钱的方法interferon-α and interferon-β which can be expressed by all cells, IFN-γ is secreted

 

不用本金就能赚钱的方法by   Th1    cells,   Tc   cells,   dendritic   cells   and   NK   cells.   Also   known    as   immune

 

interferon,   IFN-γ  is   the   only   Type   II   interferon.   It   is   serologically   distinct   from

 

不用本金就能赚钱的方法Type I interferons and it is acid-labile, while the type I variants are acid-stable.

 

不用本金就能赚钱的方法IFN-γ  has       antiviral,   immunoregulatory,         and    anti-tumour      properties.    It  alters

 

transcription in up to 30 genes producing  a variety  of physiological   and cellular

 

不用本金就能赚钱的方法responses. Amongst the effects are: Increase antigen presentation of macrophages.

 

不用本金就能赚钱的方法Activate      and   increase    lysosome      activity   in   macrophages.       Suppress     Th2    cell

 

不用本金就能赚钱的方法activity.   Cause   normal   cells   to   increase   expression   of   class   I   MHC   molecules.

 

不用本金就能赚钱的方法Promotes adhesion and binding required for leukocyte migration Promotes NK cell

 

activity.   Activation   by   IFN-γ is   achieved   by  its   interaction   with   a   heterodimeric

 

receptor   consisting   of   IFNGR1   &   IFNGR2   (interferon  gamma   receptors).   IFN-γ

 

不用本金就能赚钱的方法binding     to  the   receptor    activates   the   JAK-STAT        pathway.     In   addition,   IFN-γ

 

activates APCs and promotes Th1 differentiation by upregulating the transcription

 

factor   T-bet.    IFN-γ  is    the   hallmark    cytokine   of   Th1    cells   (whereas    Th2    cells

 

produce IL-4 and Th17 cells produce IL-17). NK cells and CD8+ cytotoxic T cells

 

不用本金就能赚钱的方法also   produce   IFN-γ.   IFN-γ suppresses   osteoclast   formation   by  rapidly  degrading

 

不用本金就能赚钱的方法the   RANK   adaptor   protein   TRAF6            in   the   RANK-RANKL   signaling            pathway,

 

which otherwise stimulates the production of NFκB.

 

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PRINCIPLE OF THE ASSAY

 

The   microtiter   plate   provided   in   this   kit   has   been  pre-coated   with   an   antibody

 

不用本金就能赚钱的方法specific    to  IFN-γ.   Standards    or  samples    are  then  added    to  the  appropriate

 

microtiter   plate   wells   with   a   biotin-conjugated   polyclonal   antibody   preparation

 

specific   for   IFN-γ  and   Avidin   conjugated   to   Horseradish   Peroxidase   (HRP)   is

 

不用本金就能赚钱的方法added     to   each   microplate    well   and    incubated.    Then    a  TMB      (3,3’5,  5’

 

不用本金就能赚钱的方法tetramethyl-benzidine) substrate solution is added to each well. Only those wells

 

that contain IFN-γ, biotin-conjugated antibody and enzyme-conjugated Avidin will

 

不用本金就能赚钱的方法exhibit   a   change   in   color.   The   enzyme-substrate   reaction   is   terminated   by   the

 

addition    of   a  sulphuric    acid  solution    and   the  color   change    is  measured

 

spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of

 

不用本金就能赚钱的方法IFN-γ in the samples is then determined by comparing the O.D. of the samples to

 

the standard curve.

 

不用本金就能赚钱的方法DETECTION RANGE

 

不用本金就能赚钱的方法0.78 ng/ml-50 ng/ml. The standard curve concentrations used for the ELISA’s were

 

50 ng/ml, 25 ng/ml, 12.5 ng/ml, 6.25 ng/ml, 3.12 ng/ml, 1.56 ng/ml, 0.78 ng/ml.

 

不用本金就能赚钱的方法SPECIFICITY

 

This    assay  recognizes    recombinant     and  natural   human    IFN-γ.   No   significant

 

cross-reactivity or interference was observed.

 

不用本金就能赚钱的方法SENSITIVITY

 

The minimum detectable dose of human IFN-γ is typically less than 0.195 ng/ml.

 

The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as

 

不用本金就能赚钱的方法the lowest protein concentration that could be differentiated from zero.

 

                                              3


不用本金就能赚钱的方法 ----------------------- Page 4-----------------------

不用本金就能赚钱的方法MATERIALS PROVIDED

 

               Reagent                                         Quantity

 

不用本金就能赚钱的方法               Assay plate                                          1

 

不用本金就能赚钱的方法                Standard                                            2

 

不用本金就能赚钱的方法                Sample Diluent                                 1 x 20 ml

 

不用本金就能赚钱的方法               Biotin-antibody Diluent                         1 x 10 ml

 

               HRP-avidin Diluent                              1 x 10 ml

 

不用本金就能赚钱的方法               Biotin-antibody                                 1 x 120l

 

不用本金就能赚钱的方法               HRP-avidin                                      1 x 120l

 

                                                               1 x 20 ml

               Wash Buffer

                                                             (25×concentrate)

 

               TMB Substrate                                   1 x 10 ml

 

不用本金就能赚钱的方法                Stop Solution                                  1 x 10 ml

 

STORAGE

 

不用本金就能赚钱的方法1.  Unopened test kits should be stored at 2-8°C upon receipt and the microtiter

 

不用本金就能赚钱的方法    plate should be kept in a sealed bag with desiccants to minimize exposure to

 

    damp air. The test kit may be used throughout the expiration date of the kit.

 

不用本金就能赚钱的方法    Refer to the package label for the expiration date.

 

不用本金就能赚钱的方法2.  Opened test kits will remain stable until the expiring date shown, provided it is

 

不用本金就能赚钱的方法    stored as prescribed above.

 

3.  A  microtiter   plate   reader   with   a   bandwidth   of   10   nm   or   less   and   an   optical

 

    density range of 0-3 OD or greater at 450nm wavelength is acceptable for use

 

    in absorbance measurement.

 

不用本金就能赚钱的方法                                            4


不用本金就能赚钱的方法 ----------------------- Page 5-----------------------

不用本金就能赚钱的方法REAGENT PREPARATION

 

Bring all reagents to room temperature before use.

 

1.   Wash   Buffer       If   crystals   have   formed   in   the   concentrate,   warm   to   room

 

不用本金就能赚钱的方法     temperature     and   mix   gently   until  the  crystals  have  completely     dissolved.

 

     Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to

 

     prepare 500 ml of Wash Buffer.

 

2.   Standard        Reconstitute the  Standard with 1.0 ml of  Sample Diluent. This

 

     reconstitution produces a stock solution of 50 ng/ml. Allow the standard to sit

 

     for   a   minimum   of   15   minutes   with   gentle   agitation   prior   to   making   serial

 

     dilutions. The undiluted standard serves as the high standard (50 ng/ml). The

 

     Sample Diluent serves as the zero standard (0 ng/ml).

 

3.   Biotin-antibody        Dilute   to   the   working   concentration   specified   on   the   vial

 

     label using Biotin-antibody Diluent(1:100), respectively.

 

不用本金就能赚钱的方法4.   HRP-avidin         Dilute to the working concentration specified on the vial label

 

     using HRP-avidin Diluent(1:100), respectively.

 

Precaution: The Stop Solution provided with this kit is an acid solution. Wear

 

不用本金就能赚钱的方法               eye, hand, face, and clothing protection when using this material.

 

不用本金就能赚钱的方法OTHER SUPPLIES REQUIRED

 

不用本金就能赚钱的方法     Microplate     reader   capable   of  measuring     absorbance    at  450   nm,   with  the

 

     correction wavelength set at 540 nm or 570 nm.

 

不用本金就能赚钱的方法     Pipettes and pipette tips.

 

     Deionized or distilled water.

 

不用本金就能赚钱的方法     Squirt bottle, manifold dispenser, or automated microplate washer.

 

                                               5


不用本金就能赚钱的方法 ----------------------- Page 6-----------------------

不用本金就能赚钱的方法SAMPLE COLLECTION AND STORAGE

 

     Cell Culture Supernates           Remove particulates by centrifugation and assay

 

不用本金就能赚钱的方法     immediately or aliquot and store samples at -20° C. Avoid repeated

 

不用本金就能赚钱的方法     freeze-thaw cycles.

     Serum      Use a serum separator tube (SST) and allow samples to clot for 30

 

     minutes before centrifugation for 15 minutes at 1000 x g. Remove serum and

 

不用本金就能赚钱的方法     assay   immediately   or   aliquot   and   store   samples   at   -20°   C.   Avoid   repeated

 

不用本金就能赚钱的方法     freeze-thaw cycles.

 

     Plasma      Collect plasma using citrate, EDTA, or heparin as an anticoagulant.

 

不用本金就能赚钱的方法     Centrifuge for 15 minutes at 1000 x g within 30 minutes of collection. Assay

 

     immediately or aliquot and store samples at -20° C. Avoid repeated

 

不用本金就能赚钱的方法     freeze-thaw cycles.

 

Note: Grossly hemolyzed samples are not suitable for use in this assay.

 

不用本金就能赚钱的方法ASSAY PROCEDURE

 

不用本金就能赚钱的方法Bring all reagents and samples to room temperature before use. It is

recommended that all samples, standards, and controls be assayed in duplicate.

 

1.   Add 100l of Standard, Blank, or Sample per well. Cover with the adhesive

 

     strip. Incubate for 2 hours at 37° C.

 

不用本金就能赚钱的方法2.   Remove the liquid of each well, don’t wash.

3.   Add 100l of Biotin-antibody working solution to each well. Incubate for 1

 

不用本金就能赚钱的方法     hour at 37°C. Biotin-antibody working solution may appear cloudy. Warm to

 

     room temperature and mix gently until solution appears uniform.

 

不用本金就能赚钱的方法4.   Aspirate each well and wash, repeating the process three times for a total of

     three   washes.   Wash   by   filling   each   well   with   Wash   Buffer   (200l)   using   a

 

     squirt    bottle,  multi-channel     pipette,   manifold     dispenser    or  autowasher.

 

     Complete   removal   of   liquid   at   each   step   is   essential   to   good   performance.

不用本金就能赚钱的方法     After   the   last   wash,   remove   any   remaining   Wash   Buffer   by   aspirating   or

 

不用本金就能赚钱的方法     decanting. Invert the plate and blot it against clean paper towels.

 

                                               6


不用本金就能赚钱的方法 ----------------------- Page 7-----------------------

不用本金就能赚钱的方法5.   Add 100l of HRP-avidin working solution to each well. Cover the microtiter

 

     plate with a new adhesive strip. Incubate for 1 hour at 37° C.

 

不用本金就能赚钱的方法6.   Repeat the aspiration and wash three times as step 4.

 

不用本金就能赚钱的方法7.   Add 90l of TMB Substrate to each well. Incubate for 30 minutes at 37°C.

 

     Keeping the plate away from drafts and other temperature fluctuations in the

 

不用本金就能赚钱的方法     dark.

 

8.   Add   50l   of  Stop   Solution   to   each   well.   If   color   change   does   not   appear

 

不用本金就能赚钱的方法     uniform, gently tap the plate to ensure thorough mixing.

 

9.   Determine      the  optical   density   of  each   well   within   30   minutes,    using   a

 

     microplate reader set to 450 nm.

 

不用本金就能赚钱的方法CALCULATION OF RESULTS

 

Average the duplicate readings for each standard, control, and sample and subtract

 

the average zero standard optical density. Create a standard curve by reducing the

 

data using computer software capable of generating a four parameter logistic (4-PL)

 

不用本金就能赚钱的方法curve-fit.   As   an  alternative,   construct   a  standard   curve    by  plotting   the  mean

 

absorbance for each standard on the y-axis against the concentration on the x-axis

 

不用本金就能赚钱的方法and   draw   a   best   fit   curve   through   the   points   on   the   graph.   The   data   may   be

 

不用本金就能赚钱的方法linearized by plotting the log of the IFN-γ concentrations versus the log of the O.D.

 

不用本金就能赚钱的方法and the best fit line can be determined by regression analysis. This procedure will

 

produce an adequate but less precise fit of the data. If samples have been diluted,

 

不用本金就能赚钱的方法the concentration read from the standard curve must be multiplied by the dilution

 

factor.

 

LIMITATIONS OF THE PROCEDURE

 

     The kit should not be used beyond the expiration date on the kit label.

 

     Do not mix or substitute reagents with those from other lots or sources.

 

     It is important that the Calibrator Diluent selected for the standard curve be

 

     consistent with the samples being assayed.

 

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     If samples generate values higher than the highest standard, dilute the samples

 

不用本金就能赚钱的方法     with the appropriate Calibrator Diluent and repeat the assay.

 

不用本金就能赚钱的方法     Any   variation     in  Standard   Diluent,    operator,   pipetting   technique,   washing

 

不用本金就能赚钱的方法     technique, incubation time or temperature, and kit age can cause variation in

 

     binding.

 

     This assay is designed to eliminate interference by soluble receptors, binding

 

     proteins, and other factors present in biological samples. Until all factors have

 

不用本金就能赚钱的方法     been   tested   in   the   Quantikine   Immunoassay,   the   possibility   of   interference

 

     cannot be excluded.

 

TECHNICAL HINTS

 

     When mixing or reconstituting protein solutions, always avoid foaming.

 

     To avoid cross-contamination, change pipette tips between additions of each

 

     standard level, between sample additions, and between reagent additions. Also,

 

     use separate reservoirs for each reagent.

 

不用本金就能赚钱的方法     When      using  an   automated    plate   washer,   adding    a   30  second   soak   period

 

     following  the   addition   of   wash   buffer,   and/or   rotating  the   plate   180   degrees

 

不用本金就能赚钱的方法     between wash steps may improve assay precision.

 

     To ensure accurate results, proper adhesion of plate sealers during incubation

 

     steps is necessary.

 

不用本金就能赚钱的方法     Substrate   Solution   should   remain   colorless   until   added   to   the   plate.   Keep

 

     Substrate     Solution   protected    from   light.  Substrate  Solution     should   change

 

不用本金就能赚钱的方法     from colorless to gradations of blue.

 

不用本金就能赚钱的方法     Stop Solution should be added to the plate in the same order as the Substrate

 

不用本金就能赚钱的方法     Solution. The color developed in the wells will turn from blue to yellow upon

 

不用本金就能赚钱的方法     addition of the Stop Solution. Wells that are green in color indicate that the

 

     Stop Solution has not mixed thoroughly with the Substrate Solution.

 

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                 RENRENγGANRAOSUGANRAOSU(IFN-γ)MEILIANMIANYIFENXIMEILIANMIANYIFENXI

不用本金就能赚钱的方法                 RENREN GANRAOSUGANRAOSU                MEILIANMIANYIFENXIMEILIANMIANYIFENXI

 

                              SHIJIHESHUOMINGSHUSHIJIHESHUOMINGSHU

不用本金就能赚钱的方法                              SHIJIHESHUOMINGSHUSHIJIHESHUOMINGSHU

 

BENSHIJIHEJINGONGYANJIUSHIYONGBENSHIJIHEJINGONGYANJIUSHIYONG

不用本金就能赚钱的方法BENSHIJIHEJINGONGYANJIUSHIYONGBENSHIJIHEJINGONGYANJIUSHIYONG

 

CHANPINBIANHAOCHANPINBIANHAO::CSB-E04577h

CHANPINBIANHAOCHANPINBIANHAO::

 

JIANCEFANWEIJIANCEFANWEI::0.78 ng/ml - 50 ng/ml

JIANCEFANWEIJIANCEFANWEI::

 

不用本金就能赚钱的方法ZUIDIJIANCEXIANZUIDIJIANCEXIAN::0.195 ng/ml

不用本金就能赚钱的方法ZUIDIJIANCEXIANZUIDIJIANCEXIAN::

 

不用本金就能赚钱的方法TEYIXINGTEYIXING::BENSHIJIHEKETONGSHIJIANCETIANRANHUOZHONGZUDERENIFN-γ,QIEYUQITAXIANGGUANDANBAIWU

TEYIXINGTEYIXING::

 

JIAOCHAFANYING。

 

不用本金就能赚钱的方法YOUXIAOQIYOUXIAOQI::6 GEYUE

YOUXIAOQIYOUXIAOQI::

 

不用本金就能赚钱的方法YUQIYINGYONGYUQIYINGYONG::ELISA       FADINGLIANGCEDINGRENXUEQING、XUEJIANG、XIBAOPEIYANGSHANGQINGHUOQITAXIANGGUANSHENG

YUQIYINGYONGYUQIYINGYONG::

 

不用本金就能赚钱的方法WUYETIZHONGIFN-γHANLIANG。

 

SHUOMINGSHUOMING::

SHUOSHUOMINGMING::

 

1.  SHIJIHEBAOCUN:-20℃(JIAOZHANGSHIJIANBUYONGSHI);2-8℃(PINFANSHIYONGSHI)。

 

2.  NONGXIDIYEDIWENBAOCUNHUIYOUYANXICHU,XISHISHIKEZAISHUIYUZHONGJIAWENZHURONG。

 

不用本金就能赚钱的方法3.  ZHONG、YINGWENSHUOMINGSHUKENENGHUIYOUBUYIZHIZHICHU,QINGYIYINGWENSHUOMINGSHUWEIZHUN。

 

不用本金就能赚钱的方法4.  GANGKAIQIDEMEILIANBANKONGZHONGKENENGHUIHANYOUSHAOXUSHUIYANGWUZHI,CIWEIZHENGCHANGXIANXIANG,BUHUIDUISHI

 

不用本金就能赚钱的方法    YANJIEGUOZAOCHENGRENHEYINGXIANG。

 

                                      9


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GAISHUGAISHU::

GAISHUGAISHU::

 

     GANRAOSU (IFN)SHI1957 NIANBEIFAXIANDE。TASHIYILEIFENMIXINGDANBAI,JUYOUGUANGPUKANG

 

BINGDU、KANGZHONGLIUHEMIANYIDIAOJIEGONGNENG。GENJUCHANSHENGGANRAOSUXIBAOLAIYUANBUTONG、LIHUAXINGZHIHE

 

SHENGWUXUEHUOXINGDECHAYI,KEFENWEIα-1b XINGGANRAOSU、β-GANRAOSUHEγGANRAOSU。γGANRAOSU

 

 (IFN-γ)YEJIAOⅡXINGIFN,ZHUYAOYOUHUOHUAT XIBAOCHANSHENG,HUOHUANK XIBAOYEKECHANSHENGIFN-γ。

 

不用本金就能赚钱的方法RENIFN-γJIYINDINGWEIYU12 HAORANSETI,ZAIDNA SHUIPINGSHANGIFN-γJIYINYUIFN-α/βJIYIN

 

不用本金就能赚钱的方法WUTONGYUANXING。RENIFN-γ CHENGSHUFENZIYOU143 GEANJISUANZUCHENG,TANGDANBAI,YITONGYUANSHUANGTIXING

 

SHICUNZAI,FENZILIANGWEI40kDa 。IFN-γ DESHENGWUXUEZUOYONGYOUJIAOYANGEDEZHONGSHUTEYIXING,REN

 

IFN-γZHIZUOYONGYURENHUOLINGZHANGLEIDONGWUDEXIBAO。QISHENGWUXUEZUOYONGYOU:(1)YOUDAODANHEXIBAO、

 

JUSHIXIBAO、SHUTUZHUANGXIBAO、PIFUCHENGXIANWEIXIBAO、XUEGUANNEIPIXIBAO、XINGZHUANGXIBAODENGMHC Ⅱ

 

LEIKANGYUANDEBIAODA,SHIQICANYUKANGYUANTICHENGHETEYIXINGMIANYIDESHIBIEGUOCHENG。CIWAI,IFN-γKE

 

SHANGDIAONEIPIXIBAOICAM-1(CD54)BIAODA,CUJINJUSHIXIBAOFcγR BIAODA,XIETONGYOUDAOTNF

 

BINGCUJINJUSHIXIBAOSHASHANGBINGYUANWEISHENGWU。(2)CUJINLPS                          TIWAICIJIXIAOSHUB           XIBAOFENMI

 

不用本金就能赚钱的方法IgG2a,JIANGDIIgG1、IgG2b、IgG3 HEIgE  DECHANSHENG;YIZHIYOUIL-4 YOUDAODEXIAOSHUB

 

XIBAOZENGZHI,IgG1 HEIgE CHANSHENGYIJIFcεR ⅡBIAODA;CUJINSAC YOUDAODERENB XIBAODE

 

ZENGZHI。(3)XIETONGIL-2 YOUDAOLAK HUOXING,CUJINT XIBAOIL-2R BIAODA。(4)YOUDAOJIXINGQI

 

DANBAIHECHENG,YOUDAOSUIYANGXIBAOFENHUA。

 

SHIYANYUANLISHIYANYUANLI::

SHIYANYUANLISHIYANYUANLI::

 

     YONGCHUNHUADEKANGTIBAOBEIWEIKONGBAN,ZHICHENGGUXIANGZAITI,WANGBAOBEIKANGIFN-γ KANGTIDEWEIKONG

 

不用本金就能赚钱的方法ZHONGYICIJIARUBIAOBENHUOBIAOZHUNPIN、SHENGWUSUHUADEKANGIFN-γKANGTI、HRP BIAOJIDEQINHESU,

 

JINGGUOCHEDIXIDIHOUYONGDIWUTMB XIANSE。TMB ZAIGUOYANGHUAWUMEIDECUIHUAXIAZHUANHUACHENGLANSE,

 

不用本金就能赚钱的方法BINGZAISUANDEZUOYONGXIAZHUANHUACHENGZUIZHONGDEHUANGSE。YANSEDESHENQIANHEYANGPINZHONGDEIFN-γCHENGZHENGXIANGGUAN。

 

不用本金就能赚钱的方法YONGMEIBIAOYIZAI450nm BOZHANGXIACEDINGXIGUANGDU (OD ZHI),JISUANYANGPINNONGDU。

 

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----------------------- Page 11-----------------------

SHIJIHEZUCHENGJISHIJIPEIZHISHIJIHEZUCHENGJISHIJIPEIZHI::

SHIJIHEZUCHENGJISHIJIPEIZHISHIJIHEZUCHENGJISHIJIPEIZHI::

 

1.  MEILIANBANMEILIANBAN(Assay plate ):                                      YIKUAI(96 KONG)。

不用本金就能赚钱的方法    MEILIANBANMEILIANBAN

 

2.  BIAOZHUNPINBIAOZHUNPIN(Standard):                                          2 PING(DONGGANPIN)。

    BIAOZHUNPINBIAOZHUNPIN

 

不用本金就能赚钱的方法3.  YANGPINXISHIYEYANGPINXISHIYE(Sample Diluent):                                  1×20ml/PING。

    YANGPINXISHIYEYANGPINXISHIYE

 

4.  SHENGWUSUBIAOJIKANGTIXISHIYESHENGWUSUBIAOJIKANGTIXISHIYE((Biotin-antibody Diluent):)             1×10ml/PING。

不用本金就能赚钱的方法    SHENGWUSUBIAOJIKANGTIXISHIYESHENGWUSUBIAOJIKANGTIXISHIYE((                          ))

 

5.  LAGENGUOYANGHUAWUMEIBIAOJIQINHESUXISHIYELAGENGUOYANGHUAWUMEIBIAOJIQINHESUXISHIYE((HRP-avidin Diluent):) 1×10ml/PING。

    LAGENGUOYANGHUAWUMEIBIAOJIQINHESUXISHIYELAGENGUOYANGHUAWUMEIBIAOJIQINHESUXISHIYE((                        ))

 

6.  SHENGWUSUBIAOJIKANGTISHENGWUSUBIAOJIKANGTI((Biotin-antibody):)                  1×120l/PING(1:100)。

    SHENGWUSUBIAOJIKANGTISHENGWUSUBIAOJIKANGTI((                 ))

 

7.  LAGENGUOYANGHUAWUMEIBIAOJIQINHESULAGENGUOYANGHUAWUMEIBIAOJIQINHESU((HRP-avidin ):)            1×120l/PING(1:100)。

不用本金就能赚钱的方法    LAGENGUOYANGHUAWUMEIBIAOJIQINHESULAGENGUOYANGHUAWUMEIBIAOJIQINHESU((               ))

 

不用本金就能赚钱的方法8.  DIWURONGYEDIWURONGYE((TMB Substrate):)                                   1×10ml/PING。

不用本金就能赚钱的方法    DIWURONGYEDIWURONGYE((                 ))

 

不用本金就能赚钱的方法9.  NONGXIDIYENONGXIDIYE((Wash Buffer ):) 1×20ml/PING,SHIYONGSHIMEIPINGYONGZHENGLIUSHUIXISHI25 BEI。

不用本金就能赚钱的方法    NONGXIDIYENONGXIDIYE((              ))

 

10.  ZHONGZHIYEZHONGZHIYE((Stop Solution):)                        1×10ml/PING(2N H  SO4)。

不用本金就能赚钱的方法    ZHONGZHIYEZHONGZHIYE((              ))                                         2

 

XUYAOERWEITIGONGDESHIJIHEQICAIXUYAOERWEITIGONGDESHIJIHEQICAI::

不用本金就能赚钱的方法XUYAOERWEITIGONGDESHIJIHEQICAIXUYAOERWEITIGONGDESHIJIHEQICAI::

 

1.  BIAOZHUNGUIGEMEIBIAOYI

 

2.  GAOSULIXINJI

 

不用本金就能赚钱的方法3.  DIANREHENGWENPEIYANGXIANG

 

4.  GANJINGDESHIGUANHEEppendof GUAN

 

5.  XILIEKEDIAOJIEYIYEQIJIXITOU,YICIJIANCEYANGPINJIAODUOSHI,ZUIHAOYONGDUOTONGDAOYIYEQI

 

6.  ZHENGLIUSHUI,RONGLIANGPINGDENG

 

BIAOBENDECAIJIJIBAOCUNBIAOBENDECAIJIJIBAOCUN::

BIAOBENDECAIJIJIBAOCUNBIAOBENDECAIJIJIBAOCUN::

 

不用本金就能赚钱的方法1.  XUEQING:QUANXUEBIAOBENQINGYUSHIWENFANGZHI2 XIAOSHIHUO4℃GUOYEHOUYU1000   x   g LIXIN20 FEN

 

    ZHONG,QUSHANGQINGJIKEJIANCE,HUOJIANGBIAOBENFANGYU-20℃HUO-80℃BAOCUN,DANYINGBIMIANFANFUDONG

 

不用本金就能赚钱的方法    RONG。

 

                                     11


不用本金就能赚钱的方法 ----------------------- Page 12-----------------------

2.  XUEJIANG:KEYONGEDTA HUOGANSUZUOWEIKANGNINGJI,BIAOBENCAIJIHOU30 FENZHONGNEIYU2   -   8°   C

 

    1000 x g LIXIN15 FENZHONG,HUOJIANGBIAOBENFANGYU-20℃HUO-80℃BAOCUN,DANYINGBIMIANFANFUDONG

 

不用本金就能赚钱的方法    RONG。

 

3.  XIBAOPEIYANGWUSHANGQINGHUOQITASHENGWUBIAOBEN:1000   x   g LIXIN20 FENZHONG,QUSHANGQINGJIKEJIAN

 

不用本金就能赚钱的方法    CE,HUOJIANGBIAOBENFANGYU-20℃HUO-80℃BAOCUN,DANYINGBIMIANFANFUDONGRONG。

 

不用本金就能赚钱的方法ZHU:ZHU:BIAOBENRONGXUEHUIYINGXIANGZUIHOUJIANCEJIEGUOBIAOBENRONGXUEHUIYINGXIANGZUIHOUJIANCEJIEGUO,YINCIRONGXUEBIAOBENBUYIJINXINGCIXIANGJIANCE,YINCIRONGXUEBIAOBENBUYIJINXINGCIXIANGJIANCE。。

不用本金就能赚钱的方法ZHUZHU::BIAOBENRONGXUEHUIYINGXIANGZUIHOUJIANCEJIEGUOBIAOBENRONGXUEHUIYINGXIANGZUIHOUJIANCEJIEGUO,,YINCIRONGXUEBIAOBENBUYIJINXINGCIXIANGJIANCEYINCIRONGXUEBIAOBENBUYIJINXINGCIXIANGJIANCE。。

 

不用本金就能赚钱的方法BIAOBENDEXISHIYUANZEBIAOBENDEXISHIYUANZE::

不用本金就能赚钱的方法BIAOBENDEXISHIYUANZEBIAOBENDEXISHIYUANZE::

 

不用本金就能赚钱的方法SHOUXIANTONGGUOWENXIANJIANSUODEFANGSHILEJIEDAICEYANGBENDEDAZHIHANLIANG,QUEDINGSHIDANGDEXISHIBEISHU。

 

ZHIYOUXISHIZHIBIAOZHUNQUXIANDEFANWEINEI,JIANCEDEJIEGUOCAISHIZHUNQUEDE。XISHIDEGUOCHENGZHONG,YING

 

不用本金就能赚钱的方法ZUOHAOXIANGXIDEJILU。ZUIHOUJISUANNONGDUSHI,XISHILE“N”BEI,BIAOBENDENONGDUYINGZAICHENGYI“N”。

 

BIAOZHUNPINDEXISHIYUANZEBIAOZHUNPINDEXISHIYUANZE::

BIAOZHUNPINDEXISHIYUANZEBIAOZHUNPINDEXISHIYUANZE::

 

2 PING,MEIPINGLINYONGQIANYIYANGPINXISHIYEXISHIZHI1ml,GAIHAOHOUJINGZHI10 FENZHONGYISHANG,RANHOU

 

FANFUDIANDAO/CUODONGYIZHURONGJIE,QINONGDUWEI50   ng/ml,ZUOXILIEBEIBIXISHIHOU,FENBIEXISHI

 

不用本金就能赚钱的方法50 ng/ml,25 ng/ml,12.5 ng/ml,6.25 ng/ml,3.12 ng/ml,1.56 ng/ml,0.78 ng/ml,

 

YANGPINXISHIYEZHIJIEZUOWEIBIAOZHUNNONGDU0 ng/ml,LINYONGQIAN15 FENZHONGNEIPEIZHI。

 

不用本金就能赚钱的方法RUPEIZHI25   ng/ml BIAOZHUNPIN:QU0.5ml           (BUYAOSHAOYU0.5ml)50   ng/ml DESHANGSHUBIAOZHUNPIN

 

JIARUHAN0.5ml YANGPINXISHIYEDEEppendorf GUANZHONG,HUNYUNJIKE,QIYUNONGDUYICILEITUI。

 

SHENGWUSUBIAOJIKANGTIDEXISHIYUANZESHENGWUSUBIAOJIKANGTIDEXISHIYUANZE::

SHENGWUSUBIAOJIKANGTIDEXISHIYUANZESHENGWUSUBIAOJIKANGTIDEXISHIYUANZE::

 

LINYONGQIANYISHENGWUSUBIAOJIKANGTIXISHIYEXISHI,XISHIQIANGENJUYUXIANJISUANHAODEMEICISHIYANSUO

 

不用本金就能赚钱的方法XUDEZONGLIANGPEIZHI(MEIKONG100l),SHIJIPEIZHISHIYINGDUOPEIZHI0.1-0.2ml。RU10l SHENGWUSU

 

BIAOJIKANGTIJIA990l          SHENGWUSUBIAOJIKANGTIXISHIYEDEBILIPEIZHI,QINGQINGHUNYUN,ZAISHIYONGQIAN

 

YIXIAOSHINEIPEIZHI。

 

                                      12


----------------------- Page 13-----------------------

LAGENGUOYANGHUAWUMEIBIAOJIQINHESUDEXISHIYUANZELAGENGUOYANGHUAWUMEIBIAOJIQINHESUDEXISHIYUANZE::

不用本金就能赚钱的方法LAGENGUOYANGHUAWUMEIBIAOJIQINHESUDEXISHIYUANZELAGENGUOYANGHUAWUMEIBIAOJIQINHESUDEXISHIYUANZE::

 

不用本金就能赚钱的方法LINYONGQIANYILAGENGUOYANGHUAWUMEIBIAOJIQINHESUXISHIYEXISHI,XISHIQIANGENJUYUXIANJISUANHAODE

 

MEICISHIYANSUOXUDEZONGLIANGPEIZHI (MEIKONG100l),SHIJIPEIZHISHIYINGDUOPEIZHI0.1-0.2ml。RU

 

10l  LAGENGUOYANGHUAWUMEIBIAOJIQINHESUJIA 990l                     LAGENGUOYANGHUAWUMEIBIAOJIQINHESUXISHI

 

YEDEBILIPEIZHI,QINGQINGHUNYUN,ZAISHIYONGQIANYIXIAOSHINEIPEIZHI。

 

CAOZUOBUZHOUCAOZUOBUZHOU::

CAOZUOBUZHOUCAOZUOBUZHOU::

 

SHIYANKAISHIQIAN,QINGTIQIANPEIZHIHAOSUOYOUSHIJI,SHIJIHUOYANGPINXISHISHI,JUNXUHUNYUN,HUNYUN

 

不用本金就能赚钱的方法SHIJINLIANGBIMIANQIPAO。MEICIJIANCEDOUYINGGAIZUOBIAOZHUNQUXIAN。RUYANGPINNONGDUGUOGAOSHI,YONGYANGPIN

 

不用本金就能赚钱的方法XISHIYEJINXINGXISHI,YISHIYANGPINFUHESHIJIHEDEJIANCEFANWEI。

 

1.  JIAYANG:FENBIESHEKONGBAIKONG、BIAOZHUNKONG、DAICEYANGPINKONG。KONGBAIKONGJIAYANGPINXISHIYE100l,

 

    YUKONGFENBIEJIABIAOZHUNPINHUODAICEYANGPIN100l,ZHUYIBUYAOYOUQIPAO,JIAYANGJIANGYANGPINJIAYU

 

不用本金就能赚钱的方法    MEIBIAOBANKONGDIBU,JINLIANGBUCHUJIKONGBI,QINGQINGHUANGDONGHUNYUN,MEIBIAOBANJIASHANGGAIHUOFUMO,

 

不用本金就能赚钱的方法    37℃FANYING120 FENZHONG。

 

不用本金就能赚钱的方法    WEIBAOZHENGSHIYANJIEGUOYOUXIAOXING,MEICISHIYANQINGSHIYONGXINDEBIAOZHUNPINRONGYE。

 

2.  QIQUYETI,SHUAIGAN,BUYONGXIDI。MEIKONGJIASHENGWUSUBIAOJIKANGTIGONGZUOYE 100l (QU1l

 

不用本金就能赚钱的方法    SHENGWUSUBIAOJIKANGTIJIA99l  SHENGWUSUBIAOJIKANGTIXISHIYEDEBILIPEIZHI,QINGQINGHUNYUN,

 

    ZAISHIYONGQIANYIXIAOSHINEIPEIZHI),37℃,60 FENZHONG。

 

不用本金就能赚钱的方法3.  WENYU60 FENZHONGHOU,QIQUKONGNEIYETI,SHUAIGAN,XIBAN3 CI,MEICIJINPAO1-2 FENZHONG,

 

不用本金就能赚钱的方法    200l/MEIKONG,SHUAIGAN。

 

不用本金就能赚钱的方法4.  MEIKONGJIALAGENGUOYANGHUAWUMEIBIAOJIQINHESUGONGZUOYE (TONGSHENGWUSUBIAOJIKANGTIGONGZUOYE)

 

不用本金就能赚钱的方法    100l,37℃,60 FENZHONG。

 

                                      13


----------------------- Page 14-----------------------

5.  WENYU60 FENZHONGHOU,QIQUKONGNEIYETI,SHUAIGAN,XIBAN5 CI,MEICIJINPAO1-2 FENZHONG,

 

    200l/MEIKONG,SHUAIGAN。

 

6.  YIXUMEIKONGJIADIWURONGYE90l,37℃BIGUANGXIANSE ((30  FENZHONGNEI,CISHIROUYANKEJIANBIAO

                                                ((

 

    ZHUNPINDEQIAN3-4 KONGYOUMINGXIANDETIDULANSE,HOU3-4 KONGTIDUBUMINGXIAN,JIKEZHONGZHI)。

 

7.  YIXUMEIKONGJIAZHONGZHIRONGYE50l,ZHONGZHIFANYING(CISHILANSELIZHUANHUANGSE)。ZHONGZHIYEDEJIA

 

不用本金就能赚钱的方法    RUSHUNXUYINGJINLIANGYUDIWUYEDEJIARUSHUNXUXIANGTONG。WEILEBAOZHENGSHIYANJIEGUODEZHUNQUEXING,DI

 

不用本金就能赚钱的方法    WUFANYINGSHIJIANDAOHOUYINGJINKUAIJIARUZHONGZHIYE。

 

不用本金就能赚钱的方法8.  YONGMEILIANYIZAI450nm BOZHANGYIXUCELIANGGEKONGDEGUANGMIDU(OD ZHI)。ZAIJIAZHONGZHIYEHOU

 

不用本金就能赚钱的方法    15 FENZHONGYINEIJINXINGJIANCE。

 

SHIYANBEIZHUSHIYANBEIZHU

SHIYANBEIZHUSHIYANBEIZHU

 

不用本金就能赚钱的方法1.  YONGHUZAICHUCISHIYONGSHIJIHESHI,YINGJIANGGEZHONGSHIJIGUANLIXINSHUFENZHONG,YIBIANSHIJIJIZHONGDAO

 

    GUANDI。

 

不用本金就能赚钱的方法2.  MEICISHIYANLIUYIKONGZUOWEIKONGBAIDIAOLINGKONG,GAIKONGBUJIARENHESHIJI,ZHISHIZUIHOUJIADIWURONG

 

    YEJI2N H  SO4。CELIANGSHIXIANYONGCIKONGDIAOOD ZHIZHILING。

              2

 

3.  WEIFANGZHIYANGPINZHENGFA,SHIYANSHIJIANGFANYINGBANFANGYUPUYOUSHIBUDEMIBIHENEI,MEIBIAOBANJIASHANG

 

    GAIHUOFUMO。

 

4.  WEISHIYONGWANDEMEIBIAOBANHUOZHESHIJI,QINGYU2-8℃BAOCUN。BIAOZHUNPIN、SHENGWUSUBIAOJIKANGTI

 

不用本金就能赚钱的方法    GONGZUOYE、LAGENGUOYANGHUAWUMEIBIAOJIQINHESUGONGZUOYEQINGYIJUSUOXUDELIANGPEIZHISHIYONG。QING

 

    WUZHONGFUSHIYONGYIXISHIGUODEBIAOZHUNPIN、SHENGWUSUBIAOJIKANGTIGONGZUOYE、HUOLAGENGUOYANGHUAWU

 

不用本金就能赚钱的方法    MEIBIAOJIQINHESUGONGZUOYE。

 

5.  JIANYIJIANCEYANGPINSHIJUNSHESHUANGKONGCEDING,YIBAOZHENGJIANCEJIEGUODEZHUNQUEXING。

 

不用本金就能赚钱的方法                                      14


不用本金就能赚钱的方法 ----------------------- Page 15-----------------------

XIBANFANGFAXIBANFANGFA::

XIBANFANGFAXIBANFANGFA::

 

SHOUGONGXIBANFANGFA:XIQU(BUKECHUJIBANBI)HUOSHUAIDIAOMEIBIAOBANNEIDEYETI;ZAISHIYANTAISHANG

 

不用本金就能赚钱的方法PUDIANJICENGXISHUIZHI,MEIBIAOBANCHAOXIAYONGLIPAIJICI;JIANGTUIJIANDEXIDIHUANCHONGYEZHISHAO0.3ml

 

ZHURUKONGNEI,JINPAO1-2 FENZHONG。GENJUXUYAO,ZHONGFUCIGUOCHENGSHUCI。

 

不用本金就能赚钱的方法 ZIDONGXIBAN:RUGUOYOUZIDONGXIBANJI,YINGZAISHULIANSHIYONGHOUZAIYONGDAOZHENGSHISHIYANGUOCHENGZHONG。

 

JISUANJISUAN::

JISUANJISUAN::

 

不用本金就能赚钱的方法YIBIAOZHUNWUDENONGDUWEIHENGZUOBIAO(DUISHUZUOBIAO),OD ZHIWEIZONGZUOBIAO(PUTONGZUOBIAO),ZAIBANDUI

 

SHUZUOBIAOZHISHANGHUICHUBIAOZHUNQUXIAN,GENJUYANGPINDEOD  ZHIYOUBIAOZHUNQUXIANCHACHUXIANGYINGDENONGDU;

 

ZAICHENGYIXISHIBEISHU;HUOYONGBIAOZHUNWUDENONGDUYUOD  ZHIJISUANCHUBIAOZHUNQUXIANDEZHIXIANHUIGUIFANG

 

CHENGSHI,JIANGYANGPINDEOD  ZHIDAIRUFANGCHENGSHI,JISUANCHUYANGPINNONGDU,ZAICHENGYIXISHIBEISHU,JI

 

WEIYANGPINDESHIJINONGDU。

 

ZHUYISHIXIANGZHUYISHIXIANG::

ZHUYISHIXIANGZHUYISHIXIANG::

 

1.  DANGHUNHEDANBAIRONGYESHIYINGJINLIANGQINGHUAN,BIMIANQIPAO。

 

2.  XIDIGUOCHENGFEICHANGZHONGYAO,BUCHONGFENDEXIDIYIZAOCHENGJIAYANGXING。

 

不用本金就能赚钱的方法3.  YICIJIAYANGSHIJIANZUIHAOKONGZHIZAI5 FENZHONGNEI,RUBIAOBENSHULIANGDUO,TUIJIANSHIYONGPAIQIANGJIAYANG。

 

不用本金就能赚钱的方法4.  QINGMEICICEDINGDETONGSHIZUOBIAOZHUNQUXIAN,ZUIHAOZUOFUKONG。

 

5.  RUBIAOBENZHONGDAICEWUZHIHANLIANGGUOGAO,QINGXIANXISHIHOUZAICEDING,JISUANSHIQINGZUIHOUCHENGYIXISHI

 

    BEISHU。

 

6.  ZAIPEIZHIBIAOZHUNPIN、JIANCERONGYEGONGZUOYESHI,QINGYIXIANGYINGDEXISHIYEPEIZHI,BUNENGHUNXIAO。

 

7.  DIWUQINGBIGUANGBAOCUN。

 

不用本金就能赚钱的方法8.  BUYAOYONGQITASHENGCHANCHANGJIADESHIJITIHUANSHIJIHEZHONGDESHIJI。

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