Introducing the Worlds First
不用本金就能赚钱的方法“Plug and Play” Simplicity
Moxi Flow is the World’s first SMART FLOW CYTOMETER. Moxi Flow is a fully automated, cassette-based flow cytometer that combines unparalleled ease of use with the precision and accuracy normally only associated with more expensive flow cytometers. This ultra-small instrument uses patented microfluidic thin-film cassettes that enable automatic load and measure operation. The Moxi Flow is also the only flow cytometer that auto-aligns the laser to each cassette to enable highly repeatable and robust results every time. No PMT gain adjustment is required. No warm up time required. Just insert a cassette, pipette your sample, and read the results in 10 seconds.
Select from one of our standard applications (i.e., Cell Counts, Viability, Apoptosis) or run the system in open “2 Parameter Flow Cytometry” mode for the ultimate in assay flexibility. This miniature open platform can be used for your most common cell assay needs, or to develop sophisticated, custom assays right at your fingertips.
Access your FCS compatible Data with “USB-on-the-Go”
Moxi Flow generates data using the industry standard FCS 3.1 format so you can open and analyze your results using your ORFLO’s VESTIGO software (coming soon), or any other standardized flow cytometry analysis software. Data is transferred simply and easily to a PC or MAC using a mini-USB cable and the on board “USB-on-the-Go” functionality. No additional software is required to perform the data transfer.
不用本金就能赚钱的方法Incredible Performance in a Small Package
More accurate and repeatable than any other automated cell counter
Cell Count with >95% accuracy
Viability with PI
Apoptosis with Annexin V
Precise cell/particle (Coulter Principle)
Blood analysis (RBC counts, WBC counts, CD4 counts, etc.)
Somatic Cell Counts in Milk
Perform state of the art flow cytometry with combined fluorescence and impedance detection with Orflo’s Moxi Flow platform.
不用本金就能赚钱的方法Moxi Flow comes with pre-loaded applications that are optimized for each assay. New applications are always under development and will be released, free of charge, to existing Moxi Flow owners via downloadable OS updates. The current applications are:
? Viability with PI ( MoxiCyte Viability Kit Protocol)
? Apoptosis ( Moxi Flow - Annexin V-PE Staining Protocol)
? Open Flow Cytometry ( Moxi Flow - CD4-PMBC Staining Protocol)
? Size Histogram
? Fluorescent Bead Assay
Perform routine cell counts with viability with a never before combination of simplicity, accuracy, and thoroughness. Simply select the “Viability Count (PI)” app, insert a cassette, pipette your cells stained with Propidium Iodide, and let the Moxi Flow take it from there. Better than 95% accurate data is generated automatically in both a color density dot plot and ORFLO’s unique Color Viability Histogram? that displays both the live cells (green) and dead cell (red) on one, easy to read size histogram. Because the Moxi Flow measures cell volume using the Coulter principle, actual cell size is measured, not just an estimate from forward or side scatter.
MoxiCyte Viability Kit Protocol
Study more advanced mechanism of cell death using the Apoptosis app. ORFLO has optimized system performance to run with our Apoptosis Assay Kit that utilizes Biolegend’s Annexin V-PE conjugated dye. Simply prepare your cells as instructed in the Apoptosis Assay Kit, select the Apoptosis app from the Moxi Flow home page and you are automatically running sophisticated apoptosis flow cytometry assays. The Color Density Scatter Plots are easily gated on the unit and percent apoptotic cells is automatically calculated.
Moxi Flow - Annexin V-PE Staining Protocol
不用本金就能赚钱的方法 Run your Moxi Flow like a Moxi Z. The Size Histogram app runs the system using the electric impedance detection mode only. Volumetric cell counts with size discrimination are automatically generated and displayed in histogram format. Particles down to 3μm and up to 25μm (effective diameter) can be analyzed depending on the cassette selected.
Design your own flow cytometry assay by selecting flourophores that are excited at 532nm. When this app is selected, researchers have the option of running the Moxi Flow at three different sensitivity levels (Low, Default, and High) to enable the system to match the sensitivity of the fluorophore being used. Because actual cell volume is measured simultaneously with the labelled flourescent activity (instead of the traditional estimate of cell volume, i.e., forward scatter), Moxi Flow will enable an array of experiments to be conducted that have, to date, not been possible.
Moxi Flow - CD4-PMBC Staining Protocol
不用本金就能赚钱的方法 The Flourescent Bead app lets researchers run the Moxi Flow in a mode optimized for Fluorescent microspheres. ORFLO will soon be announcing the release of our proprietary Moxi-Plex? kits and corresponding user interface to allow users to perform sophisticated, quantitative, multi-plex protein assays right on the unit. The Moxi-Plex update will be offered free of charge and is scheduled to be released in 2014.
In addition to Moxi-Plex, ORFLO is actively developing applications, and corresponding reagent kits, that will be released to Moxi Flow owners free of charge. Applications currently under development include, CD4+ T cell counts, cell cycle, and Micobe- Check (an app that identifies undesired microbes in cell cultures). Please check our website for the latest Moxi-Flow firmware updates containing new applications.
When you use the new Flow Cassettes, you will enjoy the benefit of having a brand new Flow Cell, perfectly aligned, for every experiment
Higher Performance & Simplicity in One Disposable Cassette.
不用本金就能赚钱的方法The unique, patent-protected thin-film Flow cassettes provide highly accurate, repeatable cell counts, cell size, and one color fluorescence analysis in 5 to 15 seconds (depending on cassette type). Inspired by the gold-standard Coulter Principle used in high-end counting systems worldwide, the Flow cassettes move thousands of cells per test through a cell sensing zone to accurately capture the impedance-based volumetric measurement of each individual cell in the sample, while simultaneously measuring each cell fluorescently. Flow cytometry methods for cell counting of individual cells is proven to be over 95% accurate versus a 75% accuracy with image-based cell counting methods.
No system contamination and integrated pre-filter for clogging prevention.
不用本金就能赚钱的方法Flow cassettes are designed with a unique “cell sieve” to minimize cell clumping and clogging. In addition, Flow cassettes contain 100% of your sample within the cassette body, eliminating the possibility for system contamination and the corresponding need for sterilization. Cassettes are simply and easily discarded when testing is finished.
Used cassette indicators.
不用本金就能赚钱的方法Each Flow cassette offers two individual tests that can be used independently. Moxi Z and Moxi Flow systems will also alert the user if a used cassette is mistakenly inserted in the unit.
Two cassette types for maximum flexibility and performance
For optimum performance for smaller calls and microbeads, we recommend using the MF-S cassettes. For larger cells and more adherent cell lines, we recommend using the MF-M cassettes.
See www.orflo/moxiz/applications for a list of cell types and corresponding cassette types.
不用本金就能赚钱的方法How it Works.
The operating principle behind the Moxi Flow Smart Flow Cytometer is a unique combination of Coulter-style cell size determination with simultaneous fluorescence detection. As cells flow single file through the microfabricated single-use flow cell the volume of each particle is measured at the exact same time as their primary fluorescence is measured using a 532nm solid state diode laser. Thousands of cells are measured in the 10 second read time and the data are plotted in a gradient density scatter plot as Cell size (volume) vs. Fluorescence (PMT voltage). Gating is easily performed on the unit using a interactive touch display, and the resulting live/dead ratios are automatically calculated (depending on the app selected). The analyzed data can also be displayed as a two color size histogram. Total volumetric cell counts are automatically determined for each test by precisely measuring the volume of fluid being analyzed.
不用本金就能赚钱的方法Simple 3 step operation.
Select desired app, insert the cassette and close the doors.
不用本金就能赚钱的方法 Once auto-alignment is complete, open the top door and pipette 50μl of labelled sample into the cassette.
Close the top door, assays run automatically and results are generated in 10 seconds.
|Moxi Flow App Note V4||Download (4.62 MB)|
|Moxi Flow - Annexin V Staining Protocol||Download (385 KB)|
|Moxi Flow Viability Staining Protocol||Download (379 KB)|
|Moxi Flow - CD4 PBMC Staining Protocol||Download (409 KB)|
Note: Each cassette holds 2 tests. When Sample 1 is complete, simply re-insert other end of cassette into Moxi Flow, and load Sample 2.
Data can be displayed on the unit in both a color density scatter plot and a two color size histogram. Simply drag gates using the intuitive touch display for instant live/dead ratio calculations and each of the gated volumetric cell counts (i.e., total population, live population, and dead population (Viability App). The mean cell volume for the gated populations is also automatically displayed on the unit.
Results from each test are stored in the standard FCS 3:1 format and can be viewed using any flow cytometry analysis package. The actual Moxi Flow screenshots from each assay (dot plots and histograms) are also stored in bitmap format for use online. Hundreds of files can be stored on each Moxi flow and are easily transferred to a Mac or PC using USB on-the-go. No aditional software is required.