英文名称：Phosphodiesterase I from Crotalus adamanteus venom；5′-Exonuclease；Oligonucleate 5′-nucleotidohydrolase
活力定义：One Unit hydrolyzes one micromole of p-nitrophenyl thymidine-5-phosphate per minute at 25℃, pH 8.9.
产品描述：Venom exonuclease (Phosphodiesterase I) successively hydrolyzes 5'-mononucleotides from 3'-OH-terminated ribo- and deoxyribo-oligonucleotides. The enzyme has an optimal pH range of 9.8-10.4 and a molecular weight of 115 kDa. Phosphodiesterase is inhibited by reducing agents such as glutathione, cysteine and ascorbic acids. It is completely inhibited by 5mM EDTA while ATP, ADP and AMP are partial inhibitors. The enzyme has an absolute requirement for Mg2+