英文名称：Protease S Aureus；Endoproteinase Glu-C from Staphylococcus aureus V8；V8 Protease
来源：Staph aureus V8
活力定义：One Unit causes a change of 0.001 A280 nm per minute at 37℃, pH 7.8 using casein as the substrate
产品描述：Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay described by Drapeau (Methods Enzymol., 45, 469, 1976).